EPHA3 was significantly u-regulated by PTCH1 knockdown, and this was attenuated by double-PTCH/FLNB knockdown, highlighting nodular characteristics of PTCH1 knockdown; MYCN was significantly downregulated by PTCH1 knockdown: a higher prevalence of strong MYCN expression has been reported in BCC with an infiltrative growth pattern, further supporting the hypothesis that different molecular pathways contribute to the formation of BCC subtypes and that PTCH1 knockdown may be more closely associated with nodular than mBCC [37]. The gene discussed is PTCH1; the disease is skin basal cell carcinoma.