Furthermore, PD-1-0520 restored immune cytokine expression (GZMB, IFN-γ, IL-2, TNF-α) in Jurkat T cells under tumor-induced immunosuppression (Figure 4I).Scratch assays showed that PD-1-0520 treatment reduced tumor cell migration in both A375 and HCT116 cells, as indicated by the persistently wider wound area in treated groups compared with DMSO controls at 12 and 24 h (Figure 5A–D). Here, TNF is linked to neoplasm.