Notably, increased frequencies of IFNγ-expressing cells, either alone or in combination with TNF production, were found in T cell subsets following in vitro stimulation in the ME-SA group As we have described previously [18], the ME-SA group also had elevated frequencies of circulating MAITs, more of which were CD8+ MAITs, whereas CD4+ MAIT and CD4–CD8- Double Negative (DN) MAITs were significantly lower in frequency than in the ME-MM group. The gene discussed is TNF; the disease is Miyoshi myopathy.