These findings may suggest that the samples with a higher frequency of DN MAIT cells have more cytotoxic capacity, with a more frequent appearance of the CD28- population in MAITs: this is in accordance with our finding that the ME-MM group showed a non-significant trend towards higher frequency of DN MAITs (Supplementary Figure S7) as well as increased cytotoxic marker expression in MAITs and downregulation of the CD28+ MAIT subset. The gene discussed is CD28; the disease is Miyoshi myopathy.