Furthermore, we investigated the main source of MAIT subsets which expressed perforin, and identified the CD4-CD8- double negative MAIT subset as the major contributor, rather than the expected CD8+ MAIT subset, in both clinical groups (p < 0.001 in MM, p = 0.038 in SA) (Fig. 3e, middle and right). This evidence concerns the gene PRF1 and Miyoshi myopathy.