Simultaneously, leukocytes-derived EVs and platelets-derived EVs positive to Annexin V were significantly lower and higher, respectively, in T1DM + individuals compared to HCs in line with previous studies where adults with MetS showed reduced levels of CD45 + EVs [51] and higher platelet-derived EVs levels were found in T2DM subjects with IR as marker for vascular dysfunction [64] and liver pathophysiology [65], as we will discuss later. Here, ANXA5 is linked to metabolic syndrome.