Finally, IF assay was performed in SPI1-depleted and non-depleted cancer cells and found that ACSL4 expression levels increased after SPI1 depletion (Fig. 8G) In Summary, the above experiments illustrate that SPI1 negatively correlates with ACSL4 in ccRCC cell line and tissue samples. Here, SPI1 is linked to nonpapillary renal cell carcinoma.