Further treatment of USP35-overexpressing GC cells with PX-478 (HIF-1α inhibitor) showed that compared with the USP35-overexpression group, the combination group of USP35 overexpression and PX-478 had decreased lactate levels and decreased expression of ICAM1, e-selectin, HIF-1α, and p-FAK, as well as reduced colonization ability (Fig. 4F–H). This evidence concerns the gene ICAM1 and gastric cancer.