We found that compared with the USP35-knockdown group, the combination group of USP35 knockdown and STING overexpression could upregulate glucose uptake, lactate, and ATP levels in GC cells, while the combination group of USP35 knockdown and the STING inhibitor C-176 had the opposite effect (Fig. 4A–C). This evidence concerns the gene USP35 and gastric cancer.