Infection of streptomycin-pretreated NLRC4-deficient mice and heterozygous controls with S. TmWT confirmed prior observations [23]: while luminal colonization was comparable across genotypes (S1A Fig), NLRC4-deficient mice exhibited elevated S. TmWT colony forming units (CFUs) in cecum tissue and mesenteric lymph nodes (mLN) compared to heterozygous controls (S1C and S1D Fig). This evidence concerns the gene NLRC4 and infection.