To elucidate the potential biological function of LRRC1 in HCC angiogenesis, we first established stable LRRC1-overexpressing HCC-LM3 cells (with low endogenous expression) and LRRC1-knockdown HepG2 cells (with high endogenous expression) (Figure S1 and Figure 2A–C). Here, LRRC1 is linked to hepatocellular carcinoma.