MPO and myositis disease: Antineutrophil cytoplasmic antibody, anti-proteinase 3 (anti-PR-3) antibody, and anti-myeloperoxidase (anti-MPO) antibody returned negative. Antibodies to nuclear antigen were positive. Hep 2 cytoplasmic pattern was noted (cytoplasmic speckled pattern, which is often associated with antibodies to ribosomal RNA synthetase, such as Jo-1 and related myositis).