Furthermore, flow cytometric analysis of CSC markers CD44 and CD133 indicated that the combined treatment led to a more pronounced reduction in CSC-like features(Fig. S2E).The colony formation assays demonstrated that ISG15 depletion or statin monotherapy moderately reduced tumor cell survival, whereas their combination synergistically suppressed proliferation (Fig. 1D-F). The gene discussed is ISG15; the disease is neoplasm.