In this investigation, our results verified the elevated p-PTEN (Ser380) is consistent with decreased PTEN lipid-phosphatase function and therefore with increased PI3K/AKT pathway activity in the ovarian tissue of the Cyclo group coupled with a subsequent increase in the ovarian p-FOXO3a level, intensifying the incidence of POF. The gene discussed is AKT1; the disease is premature menopause.