Analysis of an independent scRNA-seq dataset replicated spatial patterns of select matrisome genes; for example, NCAN which was expressed in TC1 cells, Ast-like, and Neu-like cells in our GBM sample also showed enriched expression in sub-annotated malignant tumor cells, astrocytes, and neural clusters (Supplementary Fig. 2A-C). This evidence concerns the gene NCAN and neoplasm.