Similarly, immunofluorescence labeling showed robust upregulation of annexin-A1 protein in LE/IT regions (n = 2) from an independent cohort of additional patient-derived GBM samples (n = 3), further confirming the spatial transcriptomic enrichment of ANXA1 in invasive and infiltrative regions of aggressive brain tumors (Supplementary Fig. 14, top panel). Here, ANXA1 is linked to brain neoplasm.