Nevertheless, APP-control animals displayed a significant increase in REM sleep during the light phase (WT, 68.3 ± 3.2 min; APP-control, 77.4 ± 0.9 min), similar to recent observations in APPswe/PSEN1dE9 mice58, and a trend towards increased wakefulness (WT, 506.3 ± 14.7 min; APP-control, 523.4 ± 15.6 min; Fig. 5b) and less NREM sleep (WT, 199.5 ± 12.5 min; APP-control, 172.7 ± 8.7 min; Fig. 5b) during the dark phase, which aligns with previous reports of several AD models with APP overexpression59. This evidence concerns the gene APP and Alzheimer disease.