Moreover, ROS detection in tumor tissues revealed that both the SIRT6 overexpression and TFAM knockdown groups induced oxidative stress (Figure 10K) Immunohistochemistry (IHC) analysis of corresponding tumor tissue sections supported these findings, showing reduced positive staining for related proteins compared to the control group, consistent with the results in Figure 8G (Figure 10L-M). Here, SIRT6 is linked to neoplasm.