ARF6 and hepatocellular carcinoma: To determine whether Arf6-overexpression (OE) or constitutive activation could boost LG-EV macropinocytosis, HCC cells with stable Arf6-OE (wt, constitutively active Q67L, and dominant-negative T27N) and SFR-HCC cells with stable Arf6-knockdown (KD) were generated (Fig. 4F).