Compared to NCD-fed mice, HF-HC-fed C57BL/6 mice showed an upregulation in the expression of genes belonging to TH1, TH2, and TH17 responses, inflammation, and inflammasome pathways (Itgax, Gata3, Nlpr3, Tnf-α, and Tlr9), fatty acid metabolism (Fgf21 and CD36), and fibrosis (Col1a1 and Timp1) (Fig. 1D). This evidence concerns the gene FGF21 and hydrops fetalis.