It concluded that both the iron supplement and iron oxide nanoparticles showed positive results of these parameters when compared to the anemia group; additionally, there was a significant increase of transferrin saturation in both iron oxide NP and ferrous sulfate groups when assessed with anemia group and these values have almost no or very little significant difference when compared to the control group (Elshemy, 2018). This evidence concerns the gene TF and anemia (phenotype).