The results indicated that the levels of AST (45.22 ± 10.10 U/L), ALT (65.55 ± 8.81 U/L), total bilirubin (1.32 ± 0.14 md/dL), direct bilirubin (0.38 ± 0.16 mg/dL) and indirect bilirubin (0.83 ± 0.39 mg/dL) were the highest in positive control group showing damage to the liver due to induction with phenylhydrazine and were significantly different (p ≤ 0.05) from all the other groups representing iron deficiency anemia. Here, GPT is linked to Iron deficiency anemia.