CD163 and hepatocellular carcinoma: Our single-cell RNA sequencing analysis [8] of the monocyte-macrophage subpopulation in mouse primary HCC identified monocytes, M1, M2, and non-classical macrophages, with non-classical macrophages characterized by low Cd80 expression and an absence of Cd163 expression (Fig. 1A, S1A, S1B).