To further investigate the effects of acetylcholine on the epithelium under periodontitis environment, we performed RNA-seq analysis on HOKs under four experimental conditions: untreated control (NC), acetylcholine (ACh, 100 nM), P. gingivalis infection (Pg, MOI = 50), and combined P. gingivalis infection with acetylcholine treatment (Pg + ACh). The gene discussed is JUP; the disease is periodontitis.