Subsequent GO and KEGG enrichment analyses demonstrated differentially expressed genes were substantially participating in predominant involvement of differentially expressed genes in: chromosome segregation (Figure 7D), nuclear envelope (Figure 7E), cadherin binding (Figure 7F), and the PI3K-Akt signaling pathway (Figure 7G) which was associated with gemcitabine resistance in PC (Gu et al., 2022; Lin et al., 2023). This evidence concerns the gene CDH17 and pachyonychia congenita.