Transwell invasion assays and wound healing assays further confirmed that IL-2-stimulated NK cells suppressed the invasive and migratory capacities of all three NSCLC cell lines, and the effects were significantly attenuated by IL-35 (t-test, P < 0.05) (Figures 4A, B) (Supplementary Figures 1, 2). The gene discussed is IL2; the disease is non-small cell lung carcinoma.