To evaluate the functional impact of IL-35 on NK cell-mediated cytotoxicity, supernatants from unstimulated, IL-2, and IL-2 + IL-35-stimulated NK cells were used to culture NSCLC cell lines A549(wild-type), PC-9(19del), and H1975(L858R-T790M). This evidence concerns the gene IL2 and non-small cell lung carcinoma.