To evaluate the mechanism through which N. caninum infection regulates IL-1β, we tested MCC950 (an NLRP3 inhibitor), VX765 (a CASP1 inhibitor), and SN-50 (an NF-κB inhibitor) in THP-1 cells following infection with parental strain Nc1 and NcGRA7KO (Figures 1C–H). This evidence concerns the gene NFKB1 and infection.