In glioma, perivascular SPP1–CD44 signaling and mesenchymal programs, and the purinergic CD39–CD73 interface between microglia and tumor cells, are recurrently enriched in defined niches; spatial frameworks that quantify these arrangements have been associated with prognostic and biological stratification and therefore constitute suitable benchmarks for LR pipelines. The gene discussed is SPP1; the disease is glioma.