In the hyperthyroidism validation dataset, CXCL16 and TMEM127 achieved area under the curve (AUC) values of 0.636 and 0.591, respectively, while in the AF validation dataset, the values were 0.745 and 0.720, demonstrating their diagnostic potential for both diseases (Figures 5F,G). This evidence concerns the gene CXCL16 and hyperthyroidism.