GMNN and glioblastoma: 3D multi-FOV imaging of live murine NF1−/−; PTEN−/−; EGFRVIII (NPE) glioblastoma spheroids (26) expressing a FUCCI cell cycle reporter system (Cdt1-mCherry and Geminin-mVenus) (27) as well as a Histone H2B marker (mCerulean) and cytoplasmic GFP was performed using dOPM, with up to 10 spheroids imaged per well in 96-well plates.