Based on microenvironmental characteristics of subcutaneous tumor models, tail vein models in immunocompetent mice, and GEMM spontaneous tumor models, CD8+ T cells exhibited higher activity in tail vein injection and GEMM compared to immunocompetent subcutaneous tumor mice, while M2 macrophages associated with immunosuppression showed the opposite trend (Figure S2G). This evidence concerns the gene CD8A and neoplasm.