By administrating the secretions from tumor cells with modulated CD146 gene expression to ECs, we found that secretions from tumor cells with CD146 high expression significantly promoted endothelial VEGFR2 expression whereas secretions from cells with knockdown of CD146 had little effect on VEGFR2 (Figure 5B), and ELISA showed that the level of secreted sCD146 was markedly reduced with CD146 knockdown (Figure 5C). This evidence concerns the gene KDR and neoplasm.