To ensure the translatability of the research results to the potential of human beings, we screened multi-omics approach integrating RNA sequencing and proteomic profiling of frontal cortex and hippocampus and revealed NDUFS8 deficiency in both the frontal cortex and hippocampi of AD patients, indicating brain-wide alterations as well as consistent changes at both the mRNA and protein levels. The gene discussed is NDUFS8; the disease is Alzheimer disease.