Single-nucleus RNA sequencing (snRNA-seq) analysis of Cx3cr1<sup>Cre</sup> Fkbp5<sup>flox/flox</sup> (Fkbp5 cKO) mice in the ipsilateral hemisphere reveals enhanced interactions between stroke-VAM and endothelial cells, influencing signaling pathways that maintain BBB integrity and promote neovascularization. The gene discussed is FKBP5; the disease is stroke disorder.