In contrast, combined with 12 weeks of phloretin gavage, phloretin effectively suppressed the HFD-induced decline in T-AOC (Fig. 3D), as well as the decrease in GSH-Px enzyme activity and GSH enzyme content of mouse serum (Fig. 3E,G), and inhibited the production of peroxidation products such as GSSG and MDA (Fig. 3G,H), revealing the effective amelioration of obesity-induced OS in mice by phloretin intervention. This evidence concerns the gene GSS and obesity due to melanocortin 4 receptor deficiency.