Moreover, to validate the importance of the NSUN2-centric pathway in polarization of TAMs in vivo, we removed all macrophages in C57 mice with clodronate-liposome, and then C57 mice were injected with bladder cancer cells and untreated BMDM or siNSUN2/siNSUN2-siSOCS3 BMDM to validate the function of NSUN2 and SOCS3 in vivo (Fig. 6A, B). This evidence concerns the gene NSUN2 and urinary bladder cancer.