As a result, we can see that BEX3, RPLP0, GAPDH, TUBA1C, HILPDA, PYCR1, CDC42EP1, CBX2, SPP1, TNFAIP6, AQP9, EIF4EBP1, DEFB4A gene is significantly up‐regulated in recurrent NPC tissue, while SCIN, HSPB8, RPTN, TIMD4, C7, GPR174, ZMAT1, RASGRP3, CR1, ZNF860, ZNF813, FAM214A, RALGPS2, ANGPTL1, KMO gene is significantly down‐regulated. This evidence concerns the gene DEFB4A and nasopharyngeal carcinoma.