DSG2 differed significantly between groups (p = 0.031) and was significantly lower in dogs with preclinical versus clinical ARVC (p = 0.025).<h4>Limitations</h4>Some data were collected retrospectively, and some dogs were receiving antiarrhythmic therapy.<h4>Conclusion</h4>Serum DSG2 autoantibodies can be present in boxer dogs with preclinical and clinical ARVC and apparently healthy controls. This evidence concerns the gene DSG2 and arrhythmogenic right ventricular cardiomyopathy.