The residual serum samples were stored at ‒80°C for analysis for anti-DSG2 autoantibodies using ELISA techniques.<h4>Results</h4>Forty boxer dogs were enrolled (11 healthy controls, 10 with preclinical ARVC and 19 with clinical ARVC). Here, DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy.