One half of each LN was subjected to routine histopathology, while the other half was disaggregated and analyzed by immunocytology (Ulmer et al, 2014), allowing precise quantification of the tumor load by counting the number of disseminated, gp100-positive melanoma cells per million LN cells (expressed as disseminated cancer cell density, DCCD). This evidence concerns the gene PMEL and neoplasm.