The primary objective of our study was to elucidate the effects of nano-curcumin on continuously treated cancer cells with tamoxifen (MCF7 Tamoxifen Resistant cell line) and investigate the underlying curcumin mechanisms involving Cyclin D1, DILA1, and the PI3K/AKT/mTOR signaling pathway in both MCF7 Sensitive (MCF7-S) and MCF7 Tamoxifen Resistant (MCF7-TR) cell lines. Here, AKT1 is linked to cancer.