In patients with early-onset DM, family history of DM (OR 3.60; 95% CI 1.03–15.09; P = 0.04);use of insulin (OR 3.52; 95% CI 1.00–14.87; P = 0.05); duration and dose of insulin (ORs 4.77; 95% CI 1.09–22.34; P = 0.04); In patients with late-onset DM, DM-onset age (OR 1.12; 95% CI 1.03–1.24; P<0.01);multiple diabetic patients in the family (OR 6.13; 95% CI 1.20–37.91;P = 0.03). This evidence concerns the gene INS and diabetes mellitus.