Second, performing systematic functional experiments to elucidate the roles of the signature genes in ESCC, characterize the downstream regulatory networks of key transcription factors such as GATA2, and identify their molecular targets in ESCC pathogenesis, while simultaneously leveraging clinical datasets such as KEYNOTE-181 (84) and KEYNOTE-590 (85) to validate and calibrate the TIDE algorithm and to identify ESCC-specific immunological biomarkers. Here, GATA2 is linked to esophageal squamous cell carcinoma.