Serum alanine transaminase (ALT) and aspartate aminotransferase (AST), measured 24 h after hIRI or sham surgeries, were significantly elevated in mice from HFD + IRI group as compared to their ND fed control counterparts (ND + IRI), confirming the induction of more severe liver injury developed in fatty liver model (Rokop et al., 2024). This evidence concerns the gene GPT and Hepatic steatosis.