ACSL4 and acute kidney injury: Although ferroptosis has previously been implicated in the development of AKI of renal IRI and direct nephrotoxic injury (Linkermann et al., 2014b; Zhao Z et al., 2020), our analyses revealed no significant elevation of canonical ferroptosis markers—including 4-HNE, ACSL4 protein, or AA-PE—in kidneys at 24 h post-hIRI.