Next, we validated in vivo the presence of fibrotic astrocytes within the glial scar and found that the fraction of fibrotic astrocytes (Wnt7b+GFAP+ cells) among total reactive astrocytes (GFAP+ cells) increased over time following ischemic stroke (Figure 2G-H), which was significantly correlated with the stiffness of the glial scar (Figure 2J). Here, WNT7B is linked to ischemic stroke.