Gene Set Enrichment Analysis (GSEA) further demonstrated significant enrichment in the tumor group for pathways including TNFA_SIGNALING_VIA_NFKB, KRAS_SIGNALING, and INFLAMMATORY_RESPONSE compared to the normal group (Figure 3H), indicating a pro-tumor role for macrophages in osteosarcoma progression. Here, NFKB1 is linked to osteosarcoma.