BNIP3 and osteosarcoma: Considering the effect of GABARAP on mitophagy in osteosarcoma, we next explored the expression of several key mitophagy-associated genes, including BNIP3, PINK1, Parkin, LC3B, p62, and Beclin1, which were significantly downregulated in GABARAP knockdown cells (Figure 10F; Supplementary Figure S9A).