TNF and breast cancer: An in vivo study demonstrated that gavage with GS triterpenoid saponin extract (GST) could upregulate IL-2 and IL-4 mRNA expression and reduce TNF-α expression indicating immunomodulation in breast cancer cell lines- MCF-7, MDA-MB-231 (121) along with tumor weight reduction at dose 200mg/kg body weight per day in MCF-7 and MDA-MB-468 cell lines (122).