An integrated pan-cancer approach was employed to analyze data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), ArrayExpress, and the International Cancer Genome Consortium (ICGC ), systematically assessing the prognostic relevance, clinical associations, signaling pathways, DNA methylation patterns, immune infiltration profiles, and chemotherapeutic sensitivity linked to R3HDM4 expression. Here, R3HDM4 is linked to cancer.