To confirm the specific role of MAML1 mediated STAT3 acetylation, we introduced STAT3 K685R (non-acetylatable mutant) or STAT3 K685Q (acetylatable mutant) into MAML1 depleted HCC cells, to examine whether it can recover MAML1 deficiency mediated phenotypes. The gene discussed is MAML1; the disease is hepatocellular carcinoma.