NSUN2 and infection: At 48 hpi, m5C RNA-BS-seq revealed that although m5C positional distribution remained largely unchanged, NSUN2 depletion led to a substantial reduction in m5C abundance, more pronounced under JEV (67.98%) than CSFV (47.27%) infection (Figs 4A, 4B, S12, A and S12, B).