Higher GFAP expression during the chronic phase aligns with previous studies reporting a significant increase in GFAP expression within 12 h post-reperfusion in ischemic stroke models (Block et al. 2005), persisting through subacute and chronic stages of ischemia, adding to long-term neuronal loss and reactive gliosis (Feng et al. 2020). This evidence concerns the gene GFAP and ischemic stroke.