Current diagnostic methods primarily rely on detecting CHIKV-specific IgM and IgG antibodies; however, these methods are limited to the humoral immune phase following viral infection.To address the diagnostic gap during the window period before antibody response, we developed two antigen detection assays capable of directly detecting the CHIKV envelope protein (CHIKV-E), enabling early and rapid viral detection. Here, CD40LG is linked to viral infectious disease.