In genes related to DCM, the most frequent AAs were serine (e.g., SCN5A, MYH7), alanine (e.g., SCN5A, TNNT2), tyrosine (e.g., DSP, VCL), arginine (e.g., SCN5A, MYH7), and cysteine (e.g., MYH7, VCL). Here, TNNT2 is linked to familial dilated cardiomyopathy.