Further, we reintroduced HIF-1αinto the NAT10-depleted model (NAT10 KD SiHa) torobustly validate that NAT10 influences tumor immunity through HIF-1α.To this end, we first constructed a plasmid encoding human HIF1A for overexpression and successfully transfected itinto NAT10 KD SiHa cells, with efficient overexpressionconfirmed by Western blot analysis on protein extracts from both groups(Figure H). This evidence concerns the gene HIF1A and neoplasm.