At the end point of coculture, we separately harvestedthe adherent U14 and U14-shNAT10 tumor cells and subsequently performedCFSE-based cytotoxicity,, Calcein AM/PI-costaining,, and CCK8 assays, as described in theliteratures, to visually and quantitatively assess the NP1192-mediatedenhancement of CD8+ T cell killing capacity. The gene discussed is CD8A; the disease is neoplasm.