We next assessed whether the isoform type of tau filament impacted on nucleolar pTau accumulation in the clinical disease cohorts using four groups: AD tau (AD neuropathic changes and B score > B0, indicating the presence of neurofibrillary tangle pathology), 3-repeat tau (3R tau = Pick’s disease), 4-repeat tau (4R tau = globular glial tauopathy, progressive supranuclear palsy, and corticobasal degeneration) and those with no tau inclusions (cases with B score = B0—largely LBD, FTLD-TDP and FTLD-FUS cases). This evidence concerns the gene FUS and Alzheimer disease.