To measure hypoxia in the glioma tissue of our mouse model, we quantified HIF2α expression, together with an OLIG2 analysis that served as an indicator of tumor boundaries and infiltrative areas, based on immunostaining, as well as performed a flow cytometry-based analysis of pimonidazole (PIM) adducts in PDGFB-induced brain tumors from cohorts of Pdgfbret/ret and Pdgfbret/+ mice. Here, EPAS1 is linked to central nervous system cancer.