To interrogate if MES II cells were more prevalent in certain tumor biomes70, we explored our glioma samples in a spatial context using array-based, spatial transcriptomics RNA-sequencing (stRNA-seq) technology (10x Genomics Visium), and simultaneously visualized blood vessels, hypoxic regions, and proliferative tissue areas by immunostaining for Podocalyxin (PODXL), PIM adducts, and Ki67, respectively (Figs. 5B and S9D). This evidence concerns the gene PODXL and glioma.